Cryopreservation of rainbow trout sperm by deep-freezing

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Cryopreservation of rainbow trout sperm by deep-freezing.

Cryopreservation trials on rainbow trout (Salmo gairdneri) sperm were carried out using two basic extenders: Mounib's medium (M) and Ménézo's medium (Me) to which were added bovine serum albumin (BSA) and tellurite egg yolk (Institut Pasteur). After 10 p. 100 of DMSO was added to these different deep-freeze diluents (DD), they were mixed with the sperm and then deep-frozen into 100 microliter p...

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In the present study, we successfully cryopreserved entire blastoderms of rainbow trout, Oncorhynchus mykiss, embryos being at around midblastula stage of development. The method is a potential alternative to cryopreserving the whole fish embryo, not successful so far. key words. Blastoderm Blastomeres cryopreservation embryonic cells Trout. Cybium 2008, 32(2) suppl.: 139-141. (1) Faculty of Bi...

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Effect of Freezing Techniques, Extenders and Cryoprotectants on the Fertilization Rate of Frozen Rainbow Trout (oncorhynchus Mykiss) Sperm

Rainbow trout (Oncorhynchus mykiss) sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in t...

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Influence of Freezing on Muscles of Rainbow Trout (Oncorhynchus mykiss): A Histological and Microbiological Study

Background: Freezing is a common and ancient method for preservation of foods which is applicable both under household and industrial conditions. The objective of the study was to establish histological and microbiological changes in dorsal and abdominal muscles of rainbow trout (Oncorhynchus mykiss) after freezing once and twice. Methods: Forty-five fresh rainbow trout specimens were distribu...

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Spermatozoa of rainbow trout and chum salmon, which have no potential for motility in the testis, acquire that potential in the sperm duct. This paper demonstrates that there is little difference between the levels of sodium, potassium, calcium, magnesium, chloride and osmolality of the seminal plasma in the testis and in the sperm duct. However, the bicarbonate concentration of the seminal pla...

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ژورنال

عنوان ژورنال: Reproduction Nutrition Développement

سال: 1980

ISSN: 0181-1916

DOI: 10.1051/rnd:19801012